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bone marrow mononuclear cell isolation kit tbdscience  (Beijing Solarbio Science)


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    Beijing Solarbio Science bone marrow mononuclear cell isolation kit tbdscience
    Bone Marrow Mononuclear Cell Isolation Kit Tbdscience, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bone+marrow+mononuclear+cell+isolation+kit+tbdscience/Cat+Bone+Marrow+Mononuclear+Cell+Isolation+Solution+Kit/pmc12520683__jci-135-183761-s019-14-136-156
    Average 93 stars, based on 1 article reviews
    bone marrow mononuclear cell isolation kit tbdscience - by Bioz Stars, 2026-09
    93/100 stars

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    Recombinant:

    Article Title: Jab1 promotes immune evasion and progression in acute myeloid leukemia models under oxidative stress
    Article Snippet: Poly(I:C) (HMW) Invivogen Cat# TLRL-PIC-5 Actinomycin D Sigma-Aldrich Cat# A9415 CSN5i-3 MedChemExpress Cat# 2375740-98-8 T-5224 MedChemExpress Cat# 530141-72-1 MLN4924 MedChemExpress Cat# 905579-51-3 D-Galactose MedChemExpress Cat# 59-23-4 D-Luciferin potassium MeilunBio Cat# 115144-35-9 Dimethyl sulfoxide Sigma-Aldrich Cat# 67-68-5 9 Hoechst 33342 Sigma-Aldrich Cat# B2261 7-AAD BD Biosciences Cat# 559925 Recombinant Human IL-3 PeproTech Cat# 200-03 Recombinant Human IL-6 PeproTech Cat# 200-06 Recombinant Human SCF PeproTech Cat# 300-07 Recombinant Human TPO PeproTech Cat# 300-18 Recombinant Murine IL-3 PeproTech Cat# 213-13 Recombinant Murine IL-6 PeproTech Cat# 216-16 Recombinant Murine SCF PeproTech Cat# 250-03 RNA immunoprecipitation Assay Kit Geneseed Cat# P0102 SimpleChlP Plus Sonication Chromatin IP Kit Cell Signaling Technology Cat# 56383S Cell Meter Fluorimetric Intracellular Total ROS Activity Assay Kit AAT Bioquest Cat# 22903 ROS Assay Kit Beyotime Cat# S0033S Mouse CD117 (cKIT) Positive Selection Kit StemCell Technologies Cat# 18757 Bone marrow mononuclear cell isolation kit Tbdscience Cat# TBD2013CHU Phosphate buffer saline Gibco Cat# 8115178 Red Blood Cell Lysis Buffer Solarbio Cat# R1010 RPMI 1640 medium Gibco Cat# 8115178 Penicillin-Streptomycin Solution Biosharp Cat# BL505A MethoCult medium StemCell Technologies Cat# M3434 Puromycin ScienCell Cat# 0543 Annexin V-FITC/PI apoptosis kit MultiSciences Cat# AP101 CCK-8 Cell Proliferation and Cytotoxicity Assay Kit Solarbio Cat# CA1210 10 Fix & Perm intracellular staining kit MultiSciences Cat# GAS006/2 TRIzol reagent Takara Cat# 9109 Reverse transcription reagent kit Takara Cat# RR037A QuantiTect SYBR Green PCR kit CWBIO Cat# CW0957 Fixative Solution 4% formaldehyde Biosharp Cat# BL539A Mouse Genotyping Kit Vazyme Biotech Cat# PD101 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Color PAGE Gel Rapid Preparation Kit Enzyme organism Cat# PG112 BCA Protein concentration Assay kit Beyotime Cat# P0010S 7 11 Supplementary Figure S18 9 10 12 Supplementary Figure S1.

    RNA Immunoprecipitation:

    Article Title: Jab1 promotes immune evasion and progression in acute myeloid leukemia models under oxidative stress
    Article Snippet: Poly(I:C) (HMW) Invivogen Cat# TLRL-PIC-5 Actinomycin D Sigma-Aldrich Cat# A9415 CSN5i-3 MedChemExpress Cat# 2375740-98-8 T-5224 MedChemExpress Cat# 530141-72-1 MLN4924 MedChemExpress Cat# 905579-51-3 D-Galactose MedChemExpress Cat# 59-23-4 D-Luciferin potassium MeilunBio Cat# 115144-35-9 Dimethyl sulfoxide Sigma-Aldrich Cat# 67-68-5 9 Hoechst 33342 Sigma-Aldrich Cat# B2261 7-AAD BD Biosciences Cat# 559925 Recombinant Human IL-3 PeproTech Cat# 200-03 Recombinant Human IL-6 PeproTech Cat# 200-06 Recombinant Human SCF PeproTech Cat# 300-07 Recombinant Human TPO PeproTech Cat# 300-18 Recombinant Murine IL-3 PeproTech Cat# 213-13 Recombinant Murine IL-6 PeproTech Cat# 216-16 Recombinant Murine SCF PeproTech Cat# 250-03 RNA immunoprecipitation Assay Kit Geneseed Cat# P0102 SimpleChlP Plus Sonication Chromatin IP Kit Cell Signaling Technology Cat# 56383S Cell Meter Fluorimetric Intracellular Total ROS Activity Assay Kit AAT Bioquest Cat# 22903 ROS Assay Kit Beyotime Cat# S0033S Mouse CD117 (cKIT) Positive Selection Kit StemCell Technologies Cat# 18757 Bone marrow mononuclear cell isolation kit Tbdscience Cat# TBD2013CHU Phosphate buffer saline Gibco Cat# 8115178 Red Blood Cell Lysis Buffer Solarbio Cat# R1010 RPMI 1640 medium Gibco Cat# 8115178 Penicillin-Streptomycin Solution Biosharp Cat# BL505A MethoCult medium StemCell Technologies Cat# M3434 Puromycin ScienCell Cat# 0543 Annexin V-FITC/PI apoptosis kit MultiSciences Cat# AP101 CCK-8 Cell Proliferation and Cytotoxicity Assay Kit Solarbio Cat# CA1210 10 Fix & Perm intracellular staining kit MultiSciences Cat# GAS006/2 TRIzol reagent Takara Cat# 9109 Reverse transcription reagent kit Takara Cat# RR037A QuantiTect SYBR Green PCR kit CWBIO Cat# CW0957 Fixative Solution 4% formaldehyde Biosharp Cat# BL539A Mouse Genotyping Kit Vazyme Biotech Cat# PD101 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Color PAGE Gel Rapid Preparation Kit Enzyme organism Cat# PG112 BCA Protein concentration Assay kit Beyotime Cat# P0010S 7 11 Supplementary Figure S18 9 10 12 Supplementary Figure S1.

    Sonication:

    Article Title: Jab1 promotes immune evasion and progression in acute myeloid leukemia models under oxidative stress
    Article Snippet: Poly(I:C) (HMW) Invivogen Cat# TLRL-PIC-5 Actinomycin D Sigma-Aldrich Cat# A9415 CSN5i-3 MedChemExpress Cat# 2375740-98-8 T-5224 MedChemExpress Cat# 530141-72-1 MLN4924 MedChemExpress Cat# 905579-51-3 D-Galactose MedChemExpress Cat# 59-23-4 D-Luciferin potassium MeilunBio Cat# 115144-35-9 Dimethyl sulfoxide Sigma-Aldrich Cat# 67-68-5 9 Hoechst 33342 Sigma-Aldrich Cat# B2261 7-AAD BD Biosciences Cat# 559925 Recombinant Human IL-3 PeproTech Cat# 200-03 Recombinant Human IL-6 PeproTech Cat# 200-06 Recombinant Human SCF PeproTech Cat# 300-07 Recombinant Human TPO PeproTech Cat# 300-18 Recombinant Murine IL-3 PeproTech Cat# 213-13 Recombinant Murine IL-6 PeproTech Cat# 216-16 Recombinant Murine SCF PeproTech Cat# 250-03 RNA immunoprecipitation Assay Kit Geneseed Cat# P0102 SimpleChlP Plus Sonication Chromatin IP Kit Cell Signaling Technology Cat# 56383S Cell Meter Fluorimetric Intracellular Total ROS Activity Assay Kit AAT Bioquest Cat# 22903 ROS Assay Kit Beyotime Cat# S0033S Mouse CD117 (cKIT) Positive Selection Kit StemCell Technologies Cat# 18757 Bone marrow mononuclear cell isolation kit Tbdscience Cat# TBD2013CHU Phosphate buffer saline Gibco Cat# 8115178 Red Blood Cell Lysis Buffer Solarbio Cat# R1010 RPMI 1640 medium Gibco Cat# 8115178 Penicillin-Streptomycin Solution Biosharp Cat# BL505A MethoCult medium StemCell Technologies Cat# M3434 Puromycin ScienCell Cat# 0543 Annexin V-FITC/PI apoptosis kit MultiSciences Cat# AP101 CCK-8 Cell Proliferation and Cytotoxicity Assay Kit Solarbio Cat# CA1210 10 Fix & Perm intracellular staining kit MultiSciences Cat# GAS006/2 TRIzol reagent Takara Cat# 9109 Reverse transcription reagent kit Takara Cat# RR037A QuantiTect SYBR Green PCR kit CWBIO Cat# CW0957 Fixative Solution 4% formaldehyde Biosharp Cat# BL539A Mouse Genotyping Kit Vazyme Biotech Cat# PD101 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Color PAGE Gel Rapid Preparation Kit Enzyme organism Cat# PG112 BCA Protein concentration Assay kit Beyotime Cat# P0010S 7 11 Supplementary Figure S18 9 10 12 Supplementary Figure S1.

    Chromatin Immunoprecipitation:

    Article Title: Jab1 promotes immune evasion and progression in acute myeloid leukemia models under oxidative stress
    Article Snippet: Poly(I:C) (HMW) Invivogen Cat# TLRL-PIC-5 Actinomycin D Sigma-Aldrich Cat# A9415 CSN5i-3 MedChemExpress Cat# 2375740-98-8 T-5224 MedChemExpress Cat# 530141-72-1 MLN4924 MedChemExpress Cat# 905579-51-3 D-Galactose MedChemExpress Cat# 59-23-4 D-Luciferin potassium MeilunBio Cat# 115144-35-9 Dimethyl sulfoxide Sigma-Aldrich Cat# 67-68-5 9 Hoechst 33342 Sigma-Aldrich Cat# B2261 7-AAD BD Biosciences Cat# 559925 Recombinant Human IL-3 PeproTech Cat# 200-03 Recombinant Human IL-6 PeproTech Cat# 200-06 Recombinant Human SCF PeproTech Cat# 300-07 Recombinant Human TPO PeproTech Cat# 300-18 Recombinant Murine IL-3 PeproTech Cat# 213-13 Recombinant Murine IL-6 PeproTech Cat# 216-16 Recombinant Murine SCF PeproTech Cat# 250-03 RNA immunoprecipitation Assay Kit Geneseed Cat# P0102 SimpleChlP Plus Sonication Chromatin IP Kit Cell Signaling Technology Cat# 56383S Cell Meter Fluorimetric Intracellular Total ROS Activity Assay Kit AAT Bioquest Cat# 22903 ROS Assay Kit Beyotime Cat# S0033S Mouse CD117 (cKIT) Positive Selection Kit StemCell Technologies Cat# 18757 Bone marrow mononuclear cell isolation kit Tbdscience Cat# TBD2013CHU Phosphate buffer saline Gibco Cat# 8115178 Red Blood Cell Lysis Buffer Solarbio Cat# R1010 RPMI 1640 medium Gibco Cat# 8115178 Penicillin-Streptomycin Solution Biosharp Cat# BL505A MethoCult medium StemCell Technologies Cat# M3434 Puromycin ScienCell Cat# 0543 Annexin V-FITC/PI apoptosis kit MultiSciences Cat# AP101 CCK-8 Cell Proliferation and Cytotoxicity Assay Kit Solarbio Cat# CA1210 10 Fix & Perm intracellular staining kit MultiSciences Cat# GAS006/2 TRIzol reagent Takara Cat# 9109 Reverse transcription reagent kit Takara Cat# RR037A QuantiTect SYBR Green PCR kit CWBIO Cat# CW0957 Fixative Solution 4% formaldehyde Biosharp Cat# BL539A Mouse Genotyping Kit Vazyme Biotech Cat# PD101 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Color PAGE Gel Rapid Preparation Kit Enzyme organism Cat# PG112 BCA Protein concentration Assay kit Beyotime Cat# P0010S 7 11 Supplementary Figure S18 9 10 12 Supplementary Figure S1.

    Activity Assay:

    Article Title: Jab1 promotes immune evasion and progression in acute myeloid leukemia models under oxidative stress
    Article Snippet: Poly(I:C) (HMW) Invivogen Cat# TLRL-PIC-5 Actinomycin D Sigma-Aldrich Cat# A9415 CSN5i-3 MedChemExpress Cat# 2375740-98-8 T-5224 MedChemExpress Cat# 530141-72-1 MLN4924 MedChemExpress Cat# 905579-51-3 D-Galactose MedChemExpress Cat# 59-23-4 D-Luciferin potassium MeilunBio Cat# 115144-35-9 Dimethyl sulfoxide Sigma-Aldrich Cat# 67-68-5 9 Hoechst 33342 Sigma-Aldrich Cat# B2261 7-AAD BD Biosciences Cat# 559925 Recombinant Human IL-3 PeproTech Cat# 200-03 Recombinant Human IL-6 PeproTech Cat# 200-06 Recombinant Human SCF PeproTech Cat# 300-07 Recombinant Human TPO PeproTech Cat# 300-18 Recombinant Murine IL-3 PeproTech Cat# 213-13 Recombinant Murine IL-6 PeproTech Cat# 216-16 Recombinant Murine SCF PeproTech Cat# 250-03 RNA immunoprecipitation Assay Kit Geneseed Cat# P0102 SimpleChlP Plus Sonication Chromatin IP Kit Cell Signaling Technology Cat# 56383S Cell Meter Fluorimetric Intracellular Total ROS Activity Assay Kit AAT Bioquest Cat# 22903 ROS Assay Kit Beyotime Cat# S0033S Mouse CD117 (cKIT) Positive Selection Kit StemCell Technologies Cat# 18757 Bone marrow mononuclear cell isolation kit Tbdscience Cat# TBD2013CHU Phosphate buffer saline Gibco Cat# 8115178 Red Blood Cell Lysis Buffer Solarbio Cat# R1010 RPMI 1640 medium Gibco Cat# 8115178 Penicillin-Streptomycin Solution Biosharp Cat# BL505A MethoCult medium StemCell Technologies Cat# M3434 Puromycin ScienCell Cat# 0543 Annexin V-FITC/PI apoptosis kit MultiSciences Cat# AP101 CCK-8 Cell Proliferation and Cytotoxicity Assay Kit Solarbio Cat# CA1210 10 Fix & Perm intracellular staining kit MultiSciences Cat# GAS006/2 TRIzol reagent Takara Cat# 9109 Reverse transcription reagent kit Takara Cat# RR037A QuantiTect SYBR Green PCR kit CWBIO Cat# CW0957 Fixative Solution 4% formaldehyde Biosharp Cat# BL539A Mouse Genotyping Kit Vazyme Biotech Cat# PD101 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Color PAGE Gel Rapid Preparation Kit Enzyme organism Cat# PG112 BCA Protein concentration Assay kit Beyotime Cat# P0010S 7 11 Supplementary Figure S18 9 10 12 Supplementary Figure S1.

    ROS Assay:

    Article Title: Jab1 promotes immune evasion and progression in acute myeloid leukemia models under oxidative stress
    Article Snippet: Poly(I:C) (HMW) Invivogen Cat# TLRL-PIC-5 Actinomycin D Sigma-Aldrich Cat# A9415 CSN5i-3 MedChemExpress Cat# 2375740-98-8 T-5224 MedChemExpress Cat# 530141-72-1 MLN4924 MedChemExpress Cat# 905579-51-3 D-Galactose MedChemExpress Cat# 59-23-4 D-Luciferin potassium MeilunBio Cat# 115144-35-9 Dimethyl sulfoxide Sigma-Aldrich Cat# 67-68-5 9 Hoechst 33342 Sigma-Aldrich Cat# B2261 7-AAD BD Biosciences Cat# 559925 Recombinant Human IL-3 PeproTech Cat# 200-03 Recombinant Human IL-6 PeproTech Cat# 200-06 Recombinant Human SCF PeproTech Cat# 300-07 Recombinant Human TPO PeproTech Cat# 300-18 Recombinant Murine IL-3 PeproTech Cat# 213-13 Recombinant Murine IL-6 PeproTech Cat# 216-16 Recombinant Murine SCF PeproTech Cat# 250-03 RNA immunoprecipitation Assay Kit Geneseed Cat# P0102 SimpleChlP Plus Sonication Chromatin IP Kit Cell Signaling Technology Cat# 56383S Cell Meter Fluorimetric Intracellular Total ROS Activity Assay Kit AAT Bioquest Cat# 22903 ROS Assay Kit Beyotime Cat# S0033S Mouse CD117 (cKIT) Positive Selection Kit StemCell Technologies Cat# 18757 Bone marrow mononuclear cell isolation kit Tbdscience Cat# TBD2013CHU Phosphate buffer saline Gibco Cat# 8115178 Red Blood Cell Lysis Buffer Solarbio Cat# R1010 RPMI 1640 medium Gibco Cat# 8115178 Penicillin-Streptomycin Solution Biosharp Cat# BL505A MethoCult medium StemCell Technologies Cat# M3434 Puromycin ScienCell Cat# 0543 Annexin V-FITC/PI apoptosis kit MultiSciences Cat# AP101 CCK-8 Cell Proliferation and Cytotoxicity Assay Kit Solarbio Cat# CA1210 10 Fix & Perm intracellular staining kit MultiSciences Cat# GAS006/2 TRIzol reagent Takara Cat# 9109 Reverse transcription reagent kit Takara Cat# RR037A QuantiTect SYBR Green PCR kit CWBIO Cat# CW0957 Fixative Solution 4% formaldehyde Biosharp Cat# BL539A Mouse Genotyping Kit Vazyme Biotech Cat# PD101 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Color PAGE Gel Rapid Preparation Kit Enzyme organism Cat# PG112 BCA Protein concentration Assay kit Beyotime Cat# P0010S 7 11 Supplementary Figure S18 9 10 12 Supplementary Figure S1.

    Selection:

    Article Title: Jab1 promotes immune evasion and progression in acute myeloid leukemia models under oxidative stress
    Article Snippet: Poly(I:C) (HMW) Invivogen Cat# TLRL-PIC-5 Actinomycin D Sigma-Aldrich Cat# A9415 CSN5i-3 MedChemExpress Cat# 2375740-98-8 T-5224 MedChemExpress Cat# 530141-72-1 MLN4924 MedChemExpress Cat# 905579-51-3 D-Galactose MedChemExpress Cat# 59-23-4 D-Luciferin potassium MeilunBio Cat# 115144-35-9 Dimethyl sulfoxide Sigma-Aldrich Cat# 67-68-5 9 Hoechst 33342 Sigma-Aldrich Cat# B2261 7-AAD BD Biosciences Cat# 559925 Recombinant Human IL-3 PeproTech Cat# 200-03 Recombinant Human IL-6 PeproTech Cat# 200-06 Recombinant Human SCF PeproTech Cat# 300-07 Recombinant Human TPO PeproTech Cat# 300-18 Recombinant Murine IL-3 PeproTech Cat# 213-13 Recombinant Murine IL-6 PeproTech Cat# 216-16 Recombinant Murine SCF PeproTech Cat# 250-03 RNA immunoprecipitation Assay Kit Geneseed Cat# P0102 SimpleChlP Plus Sonication Chromatin IP Kit Cell Signaling Technology Cat# 56383S Cell Meter Fluorimetric Intracellular Total ROS Activity Assay Kit AAT Bioquest Cat# 22903 ROS Assay Kit Beyotime Cat# S0033S Mouse CD117 (cKIT) Positive Selection Kit StemCell Technologies Cat# 18757 Bone marrow mononuclear cell isolation kit Tbdscience Cat# TBD2013CHU Phosphate buffer saline Gibco Cat# 8115178 Red Blood Cell Lysis Buffer Solarbio Cat# R1010 RPMI 1640 medium Gibco Cat# 8115178 Penicillin-Streptomycin Solution Biosharp Cat# BL505A MethoCult medium StemCell Technologies Cat# M3434 Puromycin ScienCell Cat# 0543 Annexin V-FITC/PI apoptosis kit MultiSciences Cat# AP101 CCK-8 Cell Proliferation and Cytotoxicity Assay Kit Solarbio Cat# CA1210 10 Fix & Perm intracellular staining kit MultiSciences Cat# GAS006/2 TRIzol reagent Takara Cat# 9109 Reverse transcription reagent kit Takara Cat# RR037A QuantiTect SYBR Green PCR kit CWBIO Cat# CW0957 Fixative Solution 4% formaldehyde Biosharp Cat# BL539A Mouse Genotyping Kit Vazyme Biotech Cat# PD101 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Color PAGE Gel Rapid Preparation Kit Enzyme organism Cat# PG112 BCA Protein concentration Assay kit Beyotime Cat# P0010S 7 11 Supplementary Figure S18 9 10 12 Supplementary Figure S1.

    Cell Isolation:

    Article Title: Jab1 promotes immune evasion and progression in acute myeloid leukemia models under oxidative stress
    Article Snippet: Poly(I:C) (HMW) Invivogen Cat# TLRL-PIC-5 Actinomycin D Sigma-Aldrich Cat# A9415 CSN5i-3 MedChemExpress Cat# 2375740-98-8 T-5224 MedChemExpress Cat# 530141-72-1 MLN4924 MedChemExpress Cat# 905579-51-3 D-Galactose MedChemExpress Cat# 59-23-4 D-Luciferin potassium MeilunBio Cat# 115144-35-9 Dimethyl sulfoxide Sigma-Aldrich Cat# 67-68-5 9 Hoechst 33342 Sigma-Aldrich Cat# B2261 7-AAD BD Biosciences Cat# 559925 Recombinant Human IL-3 PeproTech Cat# 200-03 Recombinant Human IL-6 PeproTech Cat# 200-06 Recombinant Human SCF PeproTech Cat# 300-07 Recombinant Human TPO PeproTech Cat# 300-18 Recombinant Murine IL-3 PeproTech Cat# 213-13 Recombinant Murine IL-6 PeproTech Cat# 216-16 Recombinant Murine SCF PeproTech Cat# 250-03 RNA immunoprecipitation Assay Kit Geneseed Cat# P0102 SimpleChlP Plus Sonication Chromatin IP Kit Cell Signaling Technology Cat# 56383S Cell Meter Fluorimetric Intracellular Total ROS Activity Assay Kit AAT Bioquest Cat# 22903 ROS Assay Kit Beyotime Cat# S0033S Mouse CD117 (cKIT) Positive Selection Kit StemCell Technologies Cat# 18757 Bone marrow mononuclear cell isolation kit Tbdscience Cat# TBD2013CHU Phosphate buffer saline Gibco Cat# 8115178 Red Blood Cell Lysis Buffer Solarbio Cat# R1010 RPMI 1640 medium Gibco Cat# 8115178 Penicillin-Streptomycin Solution Biosharp Cat# BL505A MethoCult medium StemCell Technologies Cat# M3434 Puromycin ScienCell Cat# 0543 Annexin V-FITC/PI apoptosis kit MultiSciences Cat# AP101 CCK-8 Cell Proliferation and Cytotoxicity Assay Kit Solarbio Cat# CA1210 10 Fix & Perm intracellular staining kit MultiSciences Cat# GAS006/2 TRIzol reagent Takara Cat# 9109 Reverse transcription reagent kit Takara Cat# RR037A QuantiTect SYBR Green PCR kit CWBIO Cat# CW0957 Fixative Solution 4% formaldehyde Biosharp Cat# BL539A Mouse Genotyping Kit Vazyme Biotech Cat# PD101 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Color PAGE Gel Rapid Preparation Kit Enzyme organism Cat# PG112 BCA Protein concentration Assay kit Beyotime Cat# P0010S 7 11 Supplementary Figure S18 9 10 12 Supplementary Figure S1.

    Saline:

    Article Title: Jab1 promotes immune evasion and progression in acute myeloid leukemia models under oxidative stress
    Article Snippet: Poly(I:C) (HMW) Invivogen Cat# TLRL-PIC-5 Actinomycin D Sigma-Aldrich Cat# A9415 CSN5i-3 MedChemExpress Cat# 2375740-98-8 T-5224 MedChemExpress Cat# 530141-72-1 MLN4924 MedChemExpress Cat# 905579-51-3 D-Galactose MedChemExpress Cat# 59-23-4 D-Luciferin potassium MeilunBio Cat# 115144-35-9 Dimethyl sulfoxide Sigma-Aldrich Cat# 67-68-5 9 Hoechst 33342 Sigma-Aldrich Cat# B2261 7-AAD BD Biosciences Cat# 559925 Recombinant Human IL-3 PeproTech Cat# 200-03 Recombinant Human IL-6 PeproTech Cat# 200-06 Recombinant Human SCF PeproTech Cat# 300-07 Recombinant Human TPO PeproTech Cat# 300-18 Recombinant Murine IL-3 PeproTech Cat# 213-13 Recombinant Murine IL-6 PeproTech Cat# 216-16 Recombinant Murine SCF PeproTech Cat# 250-03 RNA immunoprecipitation Assay Kit Geneseed Cat# P0102 SimpleChlP Plus Sonication Chromatin IP Kit Cell Signaling Technology Cat# 56383S Cell Meter Fluorimetric Intracellular Total ROS Activity Assay Kit AAT Bioquest Cat# 22903 ROS Assay Kit Beyotime Cat# S0033S Mouse CD117 (cKIT) Positive Selection Kit StemCell Technologies Cat# 18757 Bone marrow mononuclear cell isolation kit Tbdscience Cat# TBD2013CHU Phosphate buffer saline Gibco Cat# 8115178 Red Blood Cell Lysis Buffer Solarbio Cat# R1010 RPMI 1640 medium Gibco Cat# 8115178 Penicillin-Streptomycin Solution Biosharp Cat# BL505A MethoCult medium StemCell Technologies Cat# M3434 Puromycin ScienCell Cat# 0543 Annexin V-FITC/PI apoptosis kit MultiSciences Cat# AP101 CCK-8 Cell Proliferation and Cytotoxicity Assay Kit Solarbio Cat# CA1210 10 Fix & Perm intracellular staining kit MultiSciences Cat# GAS006/2 TRIzol reagent Takara Cat# 9109 Reverse transcription reagent kit Takara Cat# RR037A QuantiTect SYBR Green PCR kit CWBIO Cat# CW0957 Fixative Solution 4% formaldehyde Biosharp Cat# BL539A Mouse Genotyping Kit Vazyme Biotech Cat# PD101 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Color PAGE Gel Rapid Preparation Kit Enzyme organism Cat# PG112 BCA Protein concentration Assay kit Beyotime Cat# P0010S 7 11 Supplementary Figure S18 9 10 12 Supplementary Figure S1.

    Red Blood Cell Lysis:

    Article Title: Jab1 promotes immune evasion and progression in acute myeloid leukemia models under oxidative stress
    Article Snippet: Poly(I:C) (HMW) Invivogen Cat# TLRL-PIC-5 Actinomycin D Sigma-Aldrich Cat# A9415 CSN5i-3 MedChemExpress Cat# 2375740-98-8 T-5224 MedChemExpress Cat# 530141-72-1 MLN4924 MedChemExpress Cat# 905579-51-3 D-Galactose MedChemExpress Cat# 59-23-4 D-Luciferin potassium MeilunBio Cat# 115144-35-9 Dimethyl sulfoxide Sigma-Aldrich Cat# 67-68-5 9 Hoechst 33342 Sigma-Aldrich Cat# B2261 7-AAD BD Biosciences Cat# 559925 Recombinant Human IL-3 PeproTech Cat# 200-03 Recombinant Human IL-6 PeproTech Cat# 200-06 Recombinant Human SCF PeproTech Cat# 300-07 Recombinant Human TPO PeproTech Cat# 300-18 Recombinant Murine IL-3 PeproTech Cat# 213-13 Recombinant Murine IL-6 PeproTech Cat# 216-16 Recombinant Murine SCF PeproTech Cat# 250-03 RNA immunoprecipitation Assay Kit Geneseed Cat# P0102 SimpleChlP Plus Sonication Chromatin IP Kit Cell Signaling Technology Cat# 56383S Cell Meter Fluorimetric Intracellular Total ROS Activity Assay Kit AAT Bioquest Cat# 22903 ROS Assay Kit Beyotime Cat# S0033S Mouse CD117 (cKIT) Positive Selection Kit StemCell Technologies Cat# 18757 Bone marrow mononuclear cell isolation kit Tbdscience Cat# TBD2013CHU Phosphate buffer saline Gibco Cat# 8115178 Red Blood Cell Lysis Buffer Solarbio Cat# R1010 RPMI 1640 medium Gibco Cat# 8115178 Penicillin-Streptomycin Solution Biosharp Cat# BL505A MethoCult medium StemCell Technologies Cat# M3434 Puromycin ScienCell Cat# 0543 Annexin V-FITC/PI apoptosis kit MultiSciences Cat# AP101 CCK-8 Cell Proliferation and Cytotoxicity Assay Kit Solarbio Cat# CA1210 10 Fix & Perm intracellular staining kit MultiSciences Cat# GAS006/2 TRIzol reagent Takara Cat# 9109 Reverse transcription reagent kit Takara Cat# RR037A QuantiTect SYBR Green PCR kit CWBIO Cat# CW0957 Fixative Solution 4% formaldehyde Biosharp Cat# BL539A Mouse Genotyping Kit Vazyme Biotech Cat# PD101 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Color PAGE Gel Rapid Preparation Kit Enzyme organism Cat# PG112 BCA Protein concentration Assay kit Beyotime Cat# P0010S 7 11 Supplementary Figure S18 9 10 12 Supplementary Figure S1.

    CCK-8 Assay:

    Article Title: Jab1 promotes immune evasion and progression in acute myeloid leukemia models under oxidative stress
    Article Snippet: Poly(I:C) (HMW) Invivogen Cat# TLRL-PIC-5 Actinomycin D Sigma-Aldrich Cat# A9415 CSN5i-3 MedChemExpress Cat# 2375740-98-8 T-5224 MedChemExpress Cat# 530141-72-1 MLN4924 MedChemExpress Cat# 905579-51-3 D-Galactose MedChemExpress Cat# 59-23-4 D-Luciferin potassium MeilunBio Cat# 115144-35-9 Dimethyl sulfoxide Sigma-Aldrich Cat# 67-68-5 9 Hoechst 33342 Sigma-Aldrich Cat# B2261 7-AAD BD Biosciences Cat# 559925 Recombinant Human IL-3 PeproTech Cat# 200-03 Recombinant Human IL-6 PeproTech Cat# 200-06 Recombinant Human SCF PeproTech Cat# 300-07 Recombinant Human TPO PeproTech Cat# 300-18 Recombinant Murine IL-3 PeproTech Cat# 213-13 Recombinant Murine IL-6 PeproTech Cat# 216-16 Recombinant Murine SCF PeproTech Cat# 250-03 RNA immunoprecipitation Assay Kit Geneseed Cat# P0102 SimpleChlP Plus Sonication Chromatin IP Kit Cell Signaling Technology Cat# 56383S Cell Meter Fluorimetric Intracellular Total ROS Activity Assay Kit AAT Bioquest Cat# 22903 ROS Assay Kit Beyotime Cat# S0033S Mouse CD117 (cKIT) Positive Selection Kit StemCell Technologies Cat# 18757 Bone marrow mononuclear cell isolation kit Tbdscience Cat# TBD2013CHU Phosphate buffer saline Gibco Cat# 8115178 Red Blood Cell Lysis Buffer Solarbio Cat# R1010 RPMI 1640 medium Gibco Cat# 8115178 Penicillin-Streptomycin Solution Biosharp Cat# BL505A MethoCult medium StemCell Technologies Cat# M3434 Puromycin ScienCell Cat# 0543 Annexin V-FITC/PI apoptosis kit MultiSciences Cat# AP101 CCK-8 Cell Proliferation and Cytotoxicity Assay Kit Solarbio Cat# CA1210 10 Fix & Perm intracellular staining kit MultiSciences Cat# GAS006/2 TRIzol reagent Takara Cat# 9109 Reverse transcription reagent kit Takara Cat# RR037A QuantiTect SYBR Green PCR kit CWBIO Cat# CW0957 Fixative Solution 4% formaldehyde Biosharp Cat# BL539A Mouse Genotyping Kit Vazyme Biotech Cat# PD101 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Color PAGE Gel Rapid Preparation Kit Enzyme organism Cat# PG112 BCA Protein concentration Assay kit Beyotime Cat# P0010S 7 11 Supplementary Figure S18 9 10 12 Supplementary Figure S1.

    Cytotoxicity Assay:

    Article Title: Jab1 promotes immune evasion and progression in acute myeloid leukemia models under oxidative stress
    Article Snippet: Poly(I:C) (HMW) Invivogen Cat# TLRL-PIC-5 Actinomycin D Sigma-Aldrich Cat# A9415 CSN5i-3 MedChemExpress Cat# 2375740-98-8 T-5224 MedChemExpress Cat# 530141-72-1 MLN4924 MedChemExpress Cat# 905579-51-3 D-Galactose MedChemExpress Cat# 59-23-4 D-Luciferin potassium MeilunBio Cat# 115144-35-9 Dimethyl sulfoxide Sigma-Aldrich Cat# 67-68-5 9 Hoechst 33342 Sigma-Aldrich Cat# B2261 7-AAD BD Biosciences Cat# 559925 Recombinant Human IL-3 PeproTech Cat# 200-03 Recombinant Human IL-6 PeproTech Cat# 200-06 Recombinant Human SCF PeproTech Cat# 300-07 Recombinant Human TPO PeproTech Cat# 300-18 Recombinant Murine IL-3 PeproTech Cat# 213-13 Recombinant Murine IL-6 PeproTech Cat# 216-16 Recombinant Murine SCF PeproTech Cat# 250-03 RNA immunoprecipitation Assay Kit Geneseed Cat# P0102 SimpleChlP Plus Sonication Chromatin IP Kit Cell Signaling Technology Cat# 56383S Cell Meter Fluorimetric Intracellular Total ROS Activity Assay Kit AAT Bioquest Cat# 22903 ROS Assay Kit Beyotime Cat# S0033S Mouse CD117 (cKIT) Positive Selection Kit StemCell Technologies Cat# 18757 Bone marrow mononuclear cell isolation kit Tbdscience Cat# TBD2013CHU Phosphate buffer saline Gibco Cat# 8115178 Red Blood Cell Lysis Buffer Solarbio Cat# R1010 RPMI 1640 medium Gibco Cat# 8115178 Penicillin-Streptomycin Solution Biosharp Cat# BL505A MethoCult medium StemCell Technologies Cat# M3434 Puromycin ScienCell Cat# 0543 Annexin V-FITC/PI apoptosis kit MultiSciences Cat# AP101 CCK-8 Cell Proliferation and Cytotoxicity Assay Kit Solarbio Cat# CA1210 10 Fix & Perm intracellular staining kit MultiSciences Cat# GAS006/2 TRIzol reagent Takara Cat# 9109 Reverse transcription reagent kit Takara Cat# RR037A QuantiTect SYBR Green PCR kit CWBIO Cat# CW0957 Fixative Solution 4% formaldehyde Biosharp Cat# BL539A Mouse Genotyping Kit Vazyme Biotech Cat# PD101 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Color PAGE Gel Rapid Preparation Kit Enzyme organism Cat# PG112 BCA Protein concentration Assay kit Beyotime Cat# P0010S 7 11 Supplementary Figure S18 9 10 12 Supplementary Figure S1.

    Staining:

    Article Title: Jab1 promotes immune evasion and progression in acute myeloid leukemia models under oxidative stress
    Article Snippet: Poly(I:C) (HMW) Invivogen Cat# TLRL-PIC-5 Actinomycin D Sigma-Aldrich Cat# A9415 CSN5i-3 MedChemExpress Cat# 2375740-98-8 T-5224 MedChemExpress Cat# 530141-72-1 MLN4924 MedChemExpress Cat# 905579-51-3 D-Galactose MedChemExpress Cat# 59-23-4 D-Luciferin potassium MeilunBio Cat# 115144-35-9 Dimethyl sulfoxide Sigma-Aldrich Cat# 67-68-5 9 Hoechst 33342 Sigma-Aldrich Cat# B2261 7-AAD BD Biosciences Cat# 559925 Recombinant Human IL-3 PeproTech Cat# 200-03 Recombinant Human IL-6 PeproTech Cat# 200-06 Recombinant Human SCF PeproTech Cat# 300-07 Recombinant Human TPO PeproTech Cat# 300-18 Recombinant Murine IL-3 PeproTech Cat# 213-13 Recombinant Murine IL-6 PeproTech Cat# 216-16 Recombinant Murine SCF PeproTech Cat# 250-03 RNA immunoprecipitation Assay Kit Geneseed Cat# P0102 SimpleChlP Plus Sonication Chromatin IP Kit Cell Signaling Technology Cat# 56383S Cell Meter Fluorimetric Intracellular Total ROS Activity Assay Kit AAT Bioquest Cat# 22903 ROS Assay Kit Beyotime Cat# S0033S Mouse CD117 (cKIT) Positive Selection Kit StemCell Technologies Cat# 18757 Bone marrow mononuclear cell isolation kit Tbdscience Cat# TBD2013CHU Phosphate buffer saline Gibco Cat# 8115178 Red Blood Cell Lysis Buffer Solarbio Cat# R1010 RPMI 1640 medium Gibco Cat# 8115178 Penicillin-Streptomycin Solution Biosharp Cat# BL505A MethoCult medium StemCell Technologies Cat# M3434 Puromycin ScienCell Cat# 0543 Annexin V-FITC/PI apoptosis kit MultiSciences Cat# AP101 CCK-8 Cell Proliferation and Cytotoxicity Assay Kit Solarbio Cat# CA1210 10 Fix & Perm intracellular staining kit MultiSciences Cat# GAS006/2 TRIzol reagent Takara Cat# 9109 Reverse transcription reagent kit Takara Cat# RR037A QuantiTect SYBR Green PCR kit CWBIO Cat# CW0957 Fixative Solution 4% formaldehyde Biosharp Cat# BL539A Mouse Genotyping Kit Vazyme Biotech Cat# PD101 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Color PAGE Gel Rapid Preparation Kit Enzyme organism Cat# PG112 BCA Protein concentration Assay kit Beyotime Cat# P0010S 7 11 Supplementary Figure S18 9 10 12 Supplementary Figure S1.

    Reverse Transcription:

    Article Title: Jab1 promotes immune evasion and progression in acute myeloid leukemia models under oxidative stress
    Article Snippet: Poly(I:C) (HMW) Invivogen Cat# TLRL-PIC-5 Actinomycin D Sigma-Aldrich Cat# A9415 CSN5i-3 MedChemExpress Cat# 2375740-98-8 T-5224 MedChemExpress Cat# 530141-72-1 MLN4924 MedChemExpress Cat# 905579-51-3 D-Galactose MedChemExpress Cat# 59-23-4 D-Luciferin potassium MeilunBio Cat# 115144-35-9 Dimethyl sulfoxide Sigma-Aldrich Cat# 67-68-5 9 Hoechst 33342 Sigma-Aldrich Cat# B2261 7-AAD BD Biosciences Cat# 559925 Recombinant Human IL-3 PeproTech Cat# 200-03 Recombinant Human IL-6 PeproTech Cat# 200-06 Recombinant Human SCF PeproTech Cat# 300-07 Recombinant Human TPO PeproTech Cat# 300-18 Recombinant Murine IL-3 PeproTech Cat# 213-13 Recombinant Murine IL-6 PeproTech Cat# 216-16 Recombinant Murine SCF PeproTech Cat# 250-03 RNA immunoprecipitation Assay Kit Geneseed Cat# P0102 SimpleChlP Plus Sonication Chromatin IP Kit Cell Signaling Technology Cat# 56383S Cell Meter Fluorimetric Intracellular Total ROS Activity Assay Kit AAT Bioquest Cat# 22903 ROS Assay Kit Beyotime Cat# S0033S Mouse CD117 (cKIT) Positive Selection Kit StemCell Technologies Cat# 18757 Bone marrow mononuclear cell isolation kit Tbdscience Cat# TBD2013CHU Phosphate buffer saline Gibco Cat# 8115178 Red Blood Cell Lysis Buffer Solarbio Cat# R1010 RPMI 1640 medium Gibco Cat# 8115178 Penicillin-Streptomycin Solution Biosharp Cat# BL505A MethoCult medium StemCell Technologies Cat# M3434 Puromycin ScienCell Cat# 0543 Annexin V-FITC/PI apoptosis kit MultiSciences Cat# AP101 CCK-8 Cell Proliferation and Cytotoxicity Assay Kit Solarbio Cat# CA1210 10 Fix & Perm intracellular staining kit MultiSciences Cat# GAS006/2 TRIzol reagent Takara Cat# 9109 Reverse transcription reagent kit Takara Cat# RR037A QuantiTect SYBR Green PCR kit CWBIO Cat# CW0957 Fixative Solution 4% formaldehyde Biosharp Cat# BL539A Mouse Genotyping Kit Vazyme Biotech Cat# PD101 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Color PAGE Gel Rapid Preparation Kit Enzyme organism Cat# PG112 BCA Protein concentration Assay kit Beyotime Cat# P0010S 7 11 Supplementary Figure S18 9 10 12 Supplementary Figure S1.

    SYBR Green Assay:

    Article Title: Jab1 promotes immune evasion and progression in acute myeloid leukemia models under oxidative stress
    Article Snippet: Poly(I:C) (HMW) Invivogen Cat# TLRL-PIC-5 Actinomycin D Sigma-Aldrich Cat# A9415 CSN5i-3 MedChemExpress Cat# 2375740-98-8 T-5224 MedChemExpress Cat# 530141-72-1 MLN4924 MedChemExpress Cat# 905579-51-3 D-Galactose MedChemExpress Cat# 59-23-4 D-Luciferin potassium MeilunBio Cat# 115144-35-9 Dimethyl sulfoxide Sigma-Aldrich Cat# 67-68-5 9 Hoechst 33342 Sigma-Aldrich Cat# B2261 7-AAD BD Biosciences Cat# 559925 Recombinant Human IL-3 PeproTech Cat# 200-03 Recombinant Human IL-6 PeproTech Cat# 200-06 Recombinant Human SCF PeproTech Cat# 300-07 Recombinant Human TPO PeproTech Cat# 300-18 Recombinant Murine IL-3 PeproTech Cat# 213-13 Recombinant Murine IL-6 PeproTech Cat# 216-16 Recombinant Murine SCF PeproTech Cat# 250-03 RNA immunoprecipitation Assay Kit Geneseed Cat# P0102 SimpleChlP Plus Sonication Chromatin IP Kit Cell Signaling Technology Cat# 56383S Cell Meter Fluorimetric Intracellular Total ROS Activity Assay Kit AAT Bioquest Cat# 22903 ROS Assay Kit Beyotime Cat# S0033S Mouse CD117 (cKIT) Positive Selection Kit StemCell Technologies Cat# 18757 Bone marrow mononuclear cell isolation kit Tbdscience Cat# TBD2013CHU Phosphate buffer saline Gibco Cat# 8115178 Red Blood Cell Lysis Buffer Solarbio Cat# R1010 RPMI 1640 medium Gibco Cat# 8115178 Penicillin-Streptomycin Solution Biosharp Cat# BL505A MethoCult medium StemCell Technologies Cat# M3434 Puromycin ScienCell Cat# 0543 Annexin V-FITC/PI apoptosis kit MultiSciences Cat# AP101 CCK-8 Cell Proliferation and Cytotoxicity Assay Kit Solarbio Cat# CA1210 10 Fix & Perm intracellular staining kit MultiSciences Cat# GAS006/2 TRIzol reagent Takara Cat# 9109 Reverse transcription reagent kit Takara Cat# RR037A QuantiTect SYBR Green PCR kit CWBIO Cat# CW0957 Fixative Solution 4% formaldehyde Biosharp Cat# BL539A Mouse Genotyping Kit Vazyme Biotech Cat# PD101 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Color PAGE Gel Rapid Preparation Kit Enzyme organism Cat# PG112 BCA Protein concentration Assay kit Beyotime Cat# P0010S 7 11 Supplementary Figure S18 9 10 12 Supplementary Figure S1.

    Polymerase Chain Reaction:

    Article Title: Jab1 promotes immune evasion and progression in acute myeloid leukemia models under oxidative stress
    Article Snippet: Poly(I:C) (HMW) Invivogen Cat# TLRL-PIC-5 Actinomycin D Sigma-Aldrich Cat# A9415 CSN5i-3 MedChemExpress Cat# 2375740-98-8 T-5224 MedChemExpress Cat# 530141-72-1 MLN4924 MedChemExpress Cat# 905579-51-3 D-Galactose MedChemExpress Cat# 59-23-4 D-Luciferin potassium MeilunBio Cat# 115144-35-9 Dimethyl sulfoxide Sigma-Aldrich Cat# 67-68-5 9 Hoechst 33342 Sigma-Aldrich Cat# B2261 7-AAD BD Biosciences Cat# 559925 Recombinant Human IL-3 PeproTech Cat# 200-03 Recombinant Human IL-6 PeproTech Cat# 200-06 Recombinant Human SCF PeproTech Cat# 300-07 Recombinant Human TPO PeproTech Cat# 300-18 Recombinant Murine IL-3 PeproTech Cat# 213-13 Recombinant Murine IL-6 PeproTech Cat# 216-16 Recombinant Murine SCF PeproTech Cat# 250-03 RNA immunoprecipitation Assay Kit Geneseed Cat# P0102 SimpleChlP Plus Sonication Chromatin IP Kit Cell Signaling Technology Cat# 56383S Cell Meter Fluorimetric Intracellular Total ROS Activity Assay Kit AAT Bioquest Cat# 22903 ROS Assay Kit Beyotime Cat# S0033S Mouse CD117 (cKIT) Positive Selection Kit StemCell Technologies Cat# 18757 Bone marrow mononuclear cell isolation kit Tbdscience Cat# TBD2013CHU Phosphate buffer saline Gibco Cat# 8115178 Red Blood Cell Lysis Buffer Solarbio Cat# R1010 RPMI 1640 medium Gibco Cat# 8115178 Penicillin-Streptomycin Solution Biosharp Cat# BL505A MethoCult medium StemCell Technologies Cat# M3434 Puromycin ScienCell Cat# 0543 Annexin V-FITC/PI apoptosis kit MultiSciences Cat# AP101 CCK-8 Cell Proliferation and Cytotoxicity Assay Kit Solarbio Cat# CA1210 10 Fix & Perm intracellular staining kit MultiSciences Cat# GAS006/2 TRIzol reagent Takara Cat# 9109 Reverse transcription reagent kit Takara Cat# RR037A QuantiTect SYBR Green PCR kit CWBIO Cat# CW0957 Fixative Solution 4% formaldehyde Biosharp Cat# BL539A Mouse Genotyping Kit Vazyme Biotech Cat# PD101 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Color PAGE Gel Rapid Preparation Kit Enzyme organism Cat# PG112 BCA Protein concentration Assay kit Beyotime Cat# P0010S 7 11 Supplementary Figure S18 9 10 12 Supplementary Figure S1.

    Protein Extraction:

    Article Title: Jab1 promotes immune evasion and progression in acute myeloid leukemia models under oxidative stress
    Article Snippet: Poly(I:C) (HMW) Invivogen Cat# TLRL-PIC-5 Actinomycin D Sigma-Aldrich Cat# A9415 CSN5i-3 MedChemExpress Cat# 2375740-98-8 T-5224 MedChemExpress Cat# 530141-72-1 MLN4924 MedChemExpress Cat# 905579-51-3 D-Galactose MedChemExpress Cat# 59-23-4 D-Luciferin potassium MeilunBio Cat# 115144-35-9 Dimethyl sulfoxide Sigma-Aldrich Cat# 67-68-5 9 Hoechst 33342 Sigma-Aldrich Cat# B2261 7-AAD BD Biosciences Cat# 559925 Recombinant Human IL-3 PeproTech Cat# 200-03 Recombinant Human IL-6 PeproTech Cat# 200-06 Recombinant Human SCF PeproTech Cat# 300-07 Recombinant Human TPO PeproTech Cat# 300-18 Recombinant Murine IL-3 PeproTech Cat# 213-13 Recombinant Murine IL-6 PeproTech Cat# 216-16 Recombinant Murine SCF PeproTech Cat# 250-03 RNA immunoprecipitation Assay Kit Geneseed Cat# P0102 SimpleChlP Plus Sonication Chromatin IP Kit Cell Signaling Technology Cat# 56383S Cell Meter Fluorimetric Intracellular Total ROS Activity Assay Kit AAT Bioquest Cat# 22903 ROS Assay Kit Beyotime Cat# S0033S Mouse CD117 (cKIT) Positive Selection Kit StemCell Technologies Cat# 18757 Bone marrow mononuclear cell isolation kit Tbdscience Cat# TBD2013CHU Phosphate buffer saline Gibco Cat# 8115178 Red Blood Cell Lysis Buffer Solarbio Cat# R1010 RPMI 1640 medium Gibco Cat# 8115178 Penicillin-Streptomycin Solution Biosharp Cat# BL505A MethoCult medium StemCell Technologies Cat# M3434 Puromycin ScienCell Cat# 0543 Annexin V-FITC/PI apoptosis kit MultiSciences Cat# AP101 CCK-8 Cell Proliferation and Cytotoxicity Assay Kit Solarbio Cat# CA1210 10 Fix & Perm intracellular staining kit MultiSciences Cat# GAS006/2 TRIzol reagent Takara Cat# 9109 Reverse transcription reagent kit Takara Cat# RR037A QuantiTect SYBR Green PCR kit CWBIO Cat# CW0957 Fixative Solution 4% formaldehyde Biosharp Cat# BL539A Mouse Genotyping Kit Vazyme Biotech Cat# PD101 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Color PAGE Gel Rapid Preparation Kit Enzyme organism Cat# PG112 BCA Protein concentration Assay kit Beyotime Cat# P0010S 7 11 Supplementary Figure S18 9 10 12 Supplementary Figure S1.

    Polyacrylamide Gel Electrophoresis:

    Article Title: Jab1 promotes immune evasion and progression in acute myeloid leukemia models under oxidative stress
    Article Snippet: Poly(I:C) (HMW) Invivogen Cat# TLRL-PIC-5 Actinomycin D Sigma-Aldrich Cat# A9415 CSN5i-3 MedChemExpress Cat# 2375740-98-8 T-5224 MedChemExpress Cat# 530141-72-1 MLN4924 MedChemExpress Cat# 905579-51-3 D-Galactose MedChemExpress Cat# 59-23-4 D-Luciferin potassium MeilunBio Cat# 115144-35-9 Dimethyl sulfoxide Sigma-Aldrich Cat# 67-68-5 9 Hoechst 33342 Sigma-Aldrich Cat# B2261 7-AAD BD Biosciences Cat# 559925 Recombinant Human IL-3 PeproTech Cat# 200-03 Recombinant Human IL-6 PeproTech Cat# 200-06 Recombinant Human SCF PeproTech Cat# 300-07 Recombinant Human TPO PeproTech Cat# 300-18 Recombinant Murine IL-3 PeproTech Cat# 213-13 Recombinant Murine IL-6 PeproTech Cat# 216-16 Recombinant Murine SCF PeproTech Cat# 250-03 RNA immunoprecipitation Assay Kit Geneseed Cat# P0102 SimpleChlP Plus Sonication Chromatin IP Kit Cell Signaling Technology Cat# 56383S Cell Meter Fluorimetric Intracellular Total ROS Activity Assay Kit AAT Bioquest Cat# 22903 ROS Assay Kit Beyotime Cat# S0033S Mouse CD117 (cKIT) Positive Selection Kit StemCell Technologies Cat# 18757 Bone marrow mononuclear cell isolation kit Tbdscience Cat# TBD2013CHU Phosphate buffer saline Gibco Cat# 8115178 Red Blood Cell Lysis Buffer Solarbio Cat# R1010 RPMI 1640 medium Gibco Cat# 8115178 Penicillin-Streptomycin Solution Biosharp Cat# BL505A MethoCult medium StemCell Technologies Cat# M3434 Puromycin ScienCell Cat# 0543 Annexin V-FITC/PI apoptosis kit MultiSciences Cat# AP101 CCK-8 Cell Proliferation and Cytotoxicity Assay Kit Solarbio Cat# CA1210 10 Fix & Perm intracellular staining kit MultiSciences Cat# GAS006/2 TRIzol reagent Takara Cat# 9109 Reverse transcription reagent kit Takara Cat# RR037A QuantiTect SYBR Green PCR kit CWBIO Cat# CW0957 Fixative Solution 4% formaldehyde Biosharp Cat# BL539A Mouse Genotyping Kit Vazyme Biotech Cat# PD101 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Color PAGE Gel Rapid Preparation Kit Enzyme organism Cat# PG112 BCA Protein concentration Assay kit Beyotime Cat# P0010S 7 11 Supplementary Figure S18 9 10 12 Supplementary Figure S1.

    Protein Concentration:

    Article Title: Jab1 promotes immune evasion and progression in acute myeloid leukemia models under oxidative stress
    Article Snippet: Poly(I:C) (HMW) Invivogen Cat# TLRL-PIC-5 Actinomycin D Sigma-Aldrich Cat# A9415 CSN5i-3 MedChemExpress Cat# 2375740-98-8 T-5224 MedChemExpress Cat# 530141-72-1 MLN4924 MedChemExpress Cat# 905579-51-3 D-Galactose MedChemExpress Cat# 59-23-4 D-Luciferin potassium MeilunBio Cat# 115144-35-9 Dimethyl sulfoxide Sigma-Aldrich Cat# 67-68-5 9 Hoechst 33342 Sigma-Aldrich Cat# B2261 7-AAD BD Biosciences Cat# 559925 Recombinant Human IL-3 PeproTech Cat# 200-03 Recombinant Human IL-6 PeproTech Cat# 200-06 Recombinant Human SCF PeproTech Cat# 300-07 Recombinant Human TPO PeproTech Cat# 300-18 Recombinant Murine IL-3 PeproTech Cat# 213-13 Recombinant Murine IL-6 PeproTech Cat# 216-16 Recombinant Murine SCF PeproTech Cat# 250-03 RNA immunoprecipitation Assay Kit Geneseed Cat# P0102 SimpleChlP Plus Sonication Chromatin IP Kit Cell Signaling Technology Cat# 56383S Cell Meter Fluorimetric Intracellular Total ROS Activity Assay Kit AAT Bioquest Cat# 22903 ROS Assay Kit Beyotime Cat# S0033S Mouse CD117 (cKIT) Positive Selection Kit StemCell Technologies Cat# 18757 Bone marrow mononuclear cell isolation kit Tbdscience Cat# TBD2013CHU Phosphate buffer saline Gibco Cat# 8115178 Red Blood Cell Lysis Buffer Solarbio Cat# R1010 RPMI 1640 medium Gibco Cat# 8115178 Penicillin-Streptomycin Solution Biosharp Cat# BL505A MethoCult medium StemCell Technologies Cat# M3434 Puromycin ScienCell Cat# 0543 Annexin V-FITC/PI apoptosis kit MultiSciences Cat# AP101 CCK-8 Cell Proliferation and Cytotoxicity Assay Kit Solarbio Cat# CA1210 10 Fix & Perm intracellular staining kit MultiSciences Cat# GAS006/2 TRIzol reagent Takara Cat# 9109 Reverse transcription reagent kit Takara Cat# RR037A QuantiTect SYBR Green PCR kit CWBIO Cat# CW0957 Fixative Solution 4% formaldehyde Biosharp Cat# BL539A Mouse Genotyping Kit Vazyme Biotech Cat# PD101 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Color PAGE Gel Rapid Preparation Kit Enzyme organism Cat# PG112 BCA Protein concentration Assay kit Beyotime Cat# P0010S 7 11 Supplementary Figure S18 9 10 12 Supplementary Figure S1.



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